Kerstin Schipper Lab: UstiGate: Next generation toolkit for advanced genetic engineering of the basidiomycete chassis Ustilago maydis
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260989 | pUG14_Lvl0_P1_Ppma1 | Level 0 part. Promoter. |
| 261037 | pUG62_Lvl0_P2_ NatR-FRTwt | Level 0 part. Nourseothricin resistance cassette with FRT sites for flippase-mediated recombination. |
| 261047 | pUG72_Lvl1_P6_NatR-FRTm8 | Level 1 part. Nourseothricin resistance cassette with FRT sites for flippase-mediated recombination. |
| 261057 | pUG82_Special LvlT_car2_HygR-FRTm4 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the car2 (um06287) locus using hygromycin resistance for selection. |
| 261058 | pUG83_Special LvlT_upp3_HygR-FRTm4 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the upp3 (um11908) locus using hygromycin resistance for selection. |
| 261059 | pUG84_Special LvlT_cco1_HygR-FRTm4 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the cco1 (um00965) locus using hygromycin resistance for selection. |
| 261060 | pUG85_Special LvlT_pep4_NatR-FRTm8 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the pep4 (um04926) locus using nourseothricin resistance for selection. |
| 261061 | pUG86_Special LvlT_ipS_CbxR | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the ipS (um00844) locus using carboxin resistance for selection. |
| 261062 | pUG87_Special LvlT_pep4_HygR-FRTm4 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the pep4 (um04926) locus using hygromycin resistance for selection. |
| 261063 | pUG88_Special LvlT_car2_NatR-FRTm8 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the car2 (um06287) locus using nourseothricin resistance for selection. |
| 261064 | pUG89_Special LvlT_upp3_NatR-FRTm8 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the upp3 (um11908) locus using nourseothricin resistance for selection. |
| 261065 | pUG90_Special LvlT_upp3_G418R-FRTm8 | Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the upp3 (um11908) locus using geneticin resistance for selection. |
| 261066 | pUG91_Lvl1_P7_mod | Modified level 1 position 7 acceptor vector enabling assembly of seven level 1 modules into a level T backbone without an additional end-linker. Modified pICH47791 vector. |
| 261067 | pUG92_LvlT_AmpR_without SspI site | Modified level T vector with the SspI restriction site removed. Modified pCAT.015 vector. |