pUG81_mScarlet-I dropout_position 2+3+4+5
(Plasmid
#261056)
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PurposeLevel 1 part. mScarlet-I expression cassette for E. coli. Required for generation of Special LvlT vectors. Replaces positions 2-5 and serves as dropout cassette for pink/white screening.
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Depositing Lab
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Depositing OrganizationHeinrich-Heine-Universitaet Duesseldorf
Located in Germany -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneDummy_P2+3+4+5
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet-I cassette
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SpeciesSynthetic
- Promoter GlpT promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (not destroyed)
- 3′ cloning site BbsI (not destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byZweng et al. 2024, doi.org/10.1007/978-1-0716-4220-7_13
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: pUX909. Please visit https://doi.org/10.64898/2026.06.11.731564 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUG81_mScarlet-I dropout_position 2+3+4+5 was a gift from Kerstin Schipper (Addgene plasmid # 261056 ; http://n2t.net/addgene:261056 ; RRID:Addgene_261056)
