pUG85_Special LvlT_pep4_NatR-FRTm8
(Plasmid
#261060)
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Purpose(Empty Backbone) Special acceptor vector for a single transcription unit featuring pink/white screening. Enables chromosomal integration at the pep4 (um04926) locus using nourseothricin resistance for selection.
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Depositing Lab
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Depositing OrganizationHeinrich-Heine-Universitaet Duesseldorf
Located in Germany -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAT.015
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Backbone manufacturerAlistair McCormick (Addgene #119558)
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: pUX425. Please visit https://doi.org/10.64898/2026.06.11.731564 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUG85_Special LvlT_pep4_NatR-FRTm8 was a gift from Kerstin Schipper (Addgene plasmid # 261060 ; http://n2t.net/addgene:261060 ; RRID:Addgene_261060)
