pLenti-EF1α-GAMT_WT-PGK-Hygro
(Plasmid
#261071)
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PurposepLenti lentiviral expression vector encoding wild-type human GAMT under a human EF1a promoter and hygromycin resistance under a PGK promoter.
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Depositing Lab
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Depositing OrganizationUniversity of Texas Southwestern Medical Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLenti-EF1alpha-DEST-PGK-hygro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGAMT
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SpeciesH. sapiens (human)
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Insert Size (bp)708
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Entrez GeneGAMT (a.k.a. CCDS2, HEL-S-20, PIG2, TP53I2)
- Promoter EF1alpha
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.09.15.676412 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-EF1α-GAMT_WT-PGK-Hygro was a gift from Samuel McBrayer (Addgene plasmid # 261071 ; http://n2t.net/addgene:261071 ; RRID:Addgene_261071) -
For your References section:
Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth. Abdullah KG, Miki K, Edgar CK, Wu SA, Xiao Y, Huang YT, Drexler R, Krishna S, Puliyappadamba VT, Tay LWR, Yuan F, Savani MR, Ghoche MT, Kotermanski SE, Traylor JI, Guo L, Gunn K, Hicks WH, Shi DD, Yalcin B, Kilian K, Tang M, Moyer M, Levitt MM, El-Shami M, Oken S, Manoj N, Gattie LC, Smith BC, Kaphle P, Shipman T, West RE 3rd, Liang C, Eshac Y, Chia TY, Patel TR, Hatanpaa KJ, Raj P, Miska JM, Ramirez DMO, Ma S, Nolin TD, Lega BC, Zinn PO, Kuhn BJ, Clark NM, Williams C, Mani DR, Gillette MA, Calzado MA, Xu L, Zacharias LG, Cai F, Ksendzovsky A, Mathews TP, Losman JA, Levi B, Barron T, Gibson JR, Richardson TE, Khan OH, Agnihotri S, Hervey-Jumper SL, Chamberland S, Monje M, Huber KM, DeBerardinis RJ, McBrayer SK. Cell. 2026 Sep 15:S0092-8674(26)01010-X. doi: 10.1016/j.cell.2026.08.037. 10.1016/j.cell.2026.08.037 PubMed 42743926