Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth.
Abdullah KG, Miki K, Edgar CK, Wu SA, Xiao Y, Huang YT, Drexler R, Krishna S, Puliyappadamba VT, Tay LWR, Yuan F, Savani MR, Ghoche MT, Kotermanski SE, Traylor JI, Guo L, Gunn K, Hicks WH, Shi DD, Yalcin B, Kilian K, Tang M, Moyer M, Levitt MM, El-Shami M, Oken S, Manoj N, Gattie LC, Smith BC, Kaphle P, Shipman T, West RE 3rd, Liang C, Eshac Y, Chia TY, Patel TR, Hatanpaa KJ, Raj P, Miska JM, Ramirez DMO, Ma S, Nolin TD, Lega BC, Zinn PO, Kuhn BJ, Clark NM, Williams C, Mani DR, Gillette MA, Calzado MA, Xu L, Zacharias LG, Cai F, Ksendzovsky A, Mathews TP, Losman JA, Levi B, Barron T, Gibson JR, Richardson TE, Khan OH, Agnihotri S, Hervey-Jumper SL, Chamberland S, Monje M, Huber KM, DeBerardinis RJ, McBrayer SK
Cell. 2026 Sep 15:S0092-8674(26)01010-X. doi: 10.1016/j.cell.2026.08.037.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 261068 | pLenti-EF1α-AGAT_WT-IRES-Neo | pLenti lentiviral expression vector encoding wild-type human AGAT linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 261071 | pLenti-EF1α-GAMT_WT-PGK-Hygro | pLenti lentiviral expression vector encoding wild-type human GAMT under a human EF1a promoter and hygromycin resistance under a PGK promoter. |
| 261072 | pLenti-EF1α-GAMT_E45S-PGK-Hygro | pLenti lentiviral expression vector encoding catalytically-null human GAMT E45S under a human EF1a promoter and hygromycin resistance under a PGK promoter. |
| 261073 | pLV-EF1α-mCherry-IRES-puro | pLenti lentiviral expression vector encoding mCherry linked to puromycin selection by an IRES under a human EF1alpha promoter. |
| 261074 | pLenti-CMV-GFP-PGK-Puro | pLenti lentiviral expression vector encoding GFP under a CMV promoter and puromycin resistance under a PGK promoter. |
| 261344 | pRDB-054_4x_crAAVS1 | Lentiviral vector encoding enAsCas12a and a four-guide casette targeting the human AAVS1 safe-harbor locus under puro selection |
| 261345 | pRDB-054_4x_crAGAT | Lentiviral vector encoding enAsCas12a and a four-guide casette targeting human AGAT under puro selection |
| 261346 | LentiCRISPRv2-GFP-sgAAVS1-1 | Lentiviral vector encoding GFP alongside Cas9 and a control gRNA targeting the AAVS1 safe harbor locus |
| 261347 | LentiCRISPRv2-GFP-sgGAMT-1 | Lentiviral vector encoding GFP alongside Cas9 and a gRNA targeting GAMT |