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pRDB-054_4x_crAGAT
(Plasmid #261345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261345 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRDB-054
  • Backbone manufacturer
    John Doench, Addgene plasmid #216096
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AGAT
  • gRNA/shRNA sequence
    GTCTGACAGGTTTATACAGTGCA, GGAGCTGTTGTCCACTTGGCGCC, ATAATTGACCTGTACGCTCGGTA, TGCTCTGCCCACTATCACTTCC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3918
  • Entrez Gene
    GATM (a.k.a. AGAT, AT, CCDS3, FRTS, FRTS1, RFS)
  • Promoter EF-1alpha
  • Tag / Fusion Protein
    • Nucleoplasmin NLS, 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.15.676412 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRDB-054_4x_crAGAT was a gift from Samuel McBrayer (Addgene plasmid # 261345 ; http://n2t.net/addgene:261345 ; RRID:Addgene_261345)

  • For your References section:

    Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth. Abdullah KG, Miki K, Edgar CK, Wu SA, Xiao Y, Huang YT, Drexler R, Krishna S, Puliyappadamba VT, Tay LWR, Yuan F, Savani MR, Ghoche MT, Kotermanski SE, Traylor JI, Guo L, Gunn K, Hicks WH, Shi DD, Yalcin B, Kilian K, Tang M, Moyer M, Levitt MM, El-Shami M, Oken S, Manoj N, Gattie LC, Smith BC, Kaphle P, Shipman T, West RE 3rd, Liang C, Eshac Y, Chia TY, Patel TR, Hatanpaa KJ, Raj P, Miska JM, Ramirez DMO, Ma S, Nolin TD, Lega BC, Zinn PO, Kuhn BJ, Clark NM, Williams C, Mani DR, Gillette MA, Calzado MA, Xu L, Zacharias LG, Cai F, Ksendzovsky A, Mathews TP, Losman JA, Levi B, Barron T, Gibson JR, Richardson TE, Khan OH, Agnihotri S, Hervey-Jumper SL, Chamberland S, Monje M, Huber KM, DeBerardinis RJ, McBrayer SK. Cell. 2026 Sep 15:S0092-8674(26)01010-X. doi: 10.1016/j.cell.2026.08.037. 10.1016/j.cell.2026.08.037 PubMed 42743926
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