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pLV-EF1α-mCherry-IRES-puro
(Plasmid #261073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261073 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLV-EF1alpha-DEST-IRES-Puro
  • Backbone manufacturer
    Savani et al (2026); Addgene plasmid # 253769
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Discosoma
  • Insert Size (bp)
    708
  • Promoter EF1alpha

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry cloned from LentiGuide mCherry, originally generated by Deepta Bhattacharya (Addgene plasmid # 217005)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.15.676412 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1α-mCherry-IRES-puro was a gift from Samuel McBrayer (Addgene plasmid # 261073 ; http://n2t.net/addgene:261073 ; RRID:Addgene_261073)

  • For your References section:

    Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth. Abdullah KG, Miki K, Edgar CK, Wu SA, Xiao Y, Savani MR, Ghoche MT, Kotermanski SE, Huang YT, Traylor JI, Guo L, Gunn K, Hicks WH, Shi DD, Tang M, Levitt MM, El-Shami M, Oken S, Manoj N, Smith BC, Puliyappadamba VT, Kaphle P, Shipman T, West RE, Liang C, Patel TR, Hatanpaa KJ, Raj P, Ma S, Ksendzovsky A, Nolin TD, Lega BC, Zinn PO, Kuhn BJ, Clark NM, Williams C, Mani DR, Gillette MA, Calzado MA, Xu L, Zacharias LG, Cai F, Mathews TP, Losman JA, Richardson TE, Levi B, Monje M, Gibson JR, Khan OH, Agnihotri S, Huber KM, Chamberland S, DeBerardinis RJ, McBrayer SK. bioRxiv [Preprint]. 2025 Sep 18:2025.09.15.676412. doi: 10.1101/2025.09.15.676412. 10.1101/2025.09.15.676412 PubMed 41000912
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