pCM34
(Plasmid
#261121)
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PurposeFor C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
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Depositing Lab
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Depositing OrganizationUniversity of Toronto
Located in Canada -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261121 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLF3FShC
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Backbone manufacturerMichael Nonet, Addgene Plasmid #153083
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namergef-1p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
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SpeciesSynthetic
- Promoter rgef-1
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2025.02.28.640866v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM34 was a gift from John Calarco (Addgene plasmid # 261121 ; http://n2t.net/addgene:261121 ; RRID:Addgene_261121) -
For your References section:
Spatio-temporal protein interaction analysis using bimolecular fluorescence complementation in C. elegans. Martin CJ, Calarco JA. bioRxiv 2025.02.28.640866 10.1101/2025.02.28.640866
