Spatio-temporal protein interaction analysis using bimolecular fluorescence complementation in C. elegans

Martin CJ, Calarco JA
bioRxiv 2025.02.28.640866 Article

Plasmids from Article

ID	Plasmid	Purpose
261121	pCM34	For C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
261122	pCM35	bFOS::VC155 and mCherry expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
261124	pCM37	bJUN::VN173 and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
261125	pCM38	For C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
261131	pCM63	For C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.
261134	pCM64	For C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
261135	pCM70	For N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
261136	pCM66	PAR-6::VC155 and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
261137	pCM67	deltaPB1 PAR-6::VC155 and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.