Skip to main content
Addgene
Open Menu

pCAG_MGYP003957870823
(Plasmid #261217)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261217 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 261217-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 261217-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MGYP003957870823-SynLnoFlanks-XTEN_HibiT_HA-tdPCP-HE0690
  • Insert Size (bp)
    1689
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-5123765/v1 for Research Square preprint.

HELMHOLTZ MUNICH has filed a patent (WO2026068725A1) disclosing the capabilities of Material, and retains ownership rights to pCAG_MGYP003957870823 Material.

Recipient agrees not to file for any intellectual property protection for Material.

Please note that any use of the Material or the HELMHOLTZ MUNICH Information for any commercial purpose — or by, on behalf of or in collaboration with any for-profit entity — requires a license from HELMHOLTZ MUNICH. To obtain such a license, please contact HELMHOLTZ MUNICH by directing your request to: [email protected].

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG_MGYP003957870823 was a gift from Wolfgang Wurst (Addgene plasmid # 261217 ; http://n2t.net/addgene:261217 ; RRID:Addgene_261217)

  • For your References section:

    Creating bottom-up RNA transfer vehicles from synthetic protein assemblies. Schuhmacher MK, Gruber C, Lang CMR, Ruijpers RMW, Mazneykova L, Beinsteiner B, Krus A, Tremmel B, Reinhardt F, Kadletz K, Ma Z, Casalta L, Cambra Bort JM, Otify DY, Balken I, Hetzel L, Merl-Pham J, Dorn T, Luchner M, Bauersachs L, Ganea K, Wieser N, Emrich A, Yagmur E, Rager K, Sagindykova G, Armbrust N, Geilenkeuser J, Westmeyer GG, Becirovic E, Biel M, Istvanffy R, Vogt Weisenhorn DM, Truong DJ, Theis FJ, Ebert G, Moretti A, Erturk A, Bahr A, Kupatt C, Jasnin M, Klymiuk N, Giesert F, Wurst W. Nature. 2026 Sep 2. doi: 10.1038/s41586-026-10952-3. 10.1038/s41586-026-10952-3 PubMed 42686908
Related items: