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Creating bottom-up RNA transfer vehicles from synthetic protein assemblies.

Schuhmacher MK, Gruber C, Lang CMR, Ruijpers RMW, Mazneykova L, Beinsteiner B, Krus A, Tremmel B, Reinhardt F, Kadletz K, Ma Z, Casalta L, Cambra Bort JM, Otify DY, Balken I, Hetzel L, Merl-Pham J, Dorn T, Luchner M, Bauersachs L, Ganea K, Wieser N, Emrich A, Yagmur E, Rager K, Sagindykova G, Armbrust N, Geilenkeuser J, Westmeyer GG, Becirovic E, Biel M, Istvanffy R, Vogt Weisenhorn DM, Truong DJ, Theis FJ, Ebert G, Moretti A, Erturk A, Bahr A, Kupatt C, Jasnin M, Klymiuk N, Giesert F, Wurst W
Nature. 2026 Sep 2. doi: 10.1038/s41586-026-10952-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
261207pCAG_STV-C8Expression of the construct for a RNA transfer vehicle
261208pCAG_STV-HE0902Expression of the construct for a RNA transfer vehicle
261209pCAG_N-SplitLuc-PP7N-SplitLuc part of firefly luciferase to be used as cargo in RNA transfer vehicles
261210pCAG_C-SplitLucGeneration of a stable expressing cell line with the C part of firefly luciferase
261211pCAG_EGFP-PP7EGFP construct to be used as cargo in RNA transfer vehicles
261212pCAG_Cas9-NLS-NES-PP7Cas9-NLS-NES construct to be used as cargo in RNA transfer vehicles
261213pCAG_STV-HE0490Expression of the construct for a RNA transfer vehicle
261214pCAG_STV-HE0499Expression of the construct for a RNA transfer vehicle
261215pCAG_STV-HE0505Expression of the construct for a RNA transfer vehicle
261216pCAG_Q9HB21Expression of the construct for a RNA transfer vehicle, containing a different membrane binding domain
261217pCAG_MGYP003957870823Expression of the construct for a RNA transfer vehicle, containing a different membrane binding domain
261218pCAG_A0A2I0TQP5Expression of the construct for a RNA transfer vehicle, containing a different membrane binding domain
261219pCAG_A0A093CX92Expression of the construct for a RNA transfer vehicle, containing a different membrane binding domain
261220pCAG_A0A6P5IML8Expression of the construct for a RNA transfer vehicle, containing a different membrane binding domain
261221pCAG_mRuby3-PP7mRuby construct to be used as cargo in RNA transfer vehicles
261222pU6_Cas9_gRNA-PP7Cas 9 gRNA cloning scaffold

Antibodies from Article