pCAG_mRuby3-PP7
(Plasmid
#261221)
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PurposemRuby construct to be used as cargo in RNA transfer vehicles
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Depositing Lab
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Depositing OrganizationHelmholtz Munich
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261221 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRuby3-NES-PP7
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Insert Size (bp)795
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-5123765/v1 for Research Square preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG_mRuby3-PP7 was a gift from Wolfgang Wurst (Addgene plasmid # 261221 ; http://n2t.net/addgene:261221 ; RRID:Addgene_261221) -
For your References section:
Creating bottom-up RNA transfer vehicles from synthetic protein assemblies. Schuhmacher MK, Gruber C, Lang CMR, Ruijpers RMW, Mazneykova L, Beinsteiner B, Krus A, Tremmel B, Reinhardt F, Kadletz K, Ma Z, Casalta L, Cambra Bort JM, Otify DY, Balken I, Hetzel L, Merl-Pham J, Dorn T, Luchner M, Bauersachs L, Ganea K, Wieser N, Emrich A, Yagmur E, Rager K, Sagindykova G, Armbrust N, Geilenkeuser J, Westmeyer GG, Becirovic E, Biel M, Istvanffy R, Vogt Weisenhorn DM, Truong DJ, Theis FJ, Ebert G, Moretti A, Erturk A, Bahr A, Kupatt C, Jasnin M, Klymiuk N, Giesert F, Wurst W. Nature. 2026 Sep 2. doi: 10.1038/s41586-026-10952-3. 10.1038/s41586-026-10952-3 PubMed 42686908