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pCAG_Cas9-NLS-NES-PP7
(Plasmid #261212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261212 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-3xNLS-FLAG-NES_PP7
  • Insert Size (bp)
    4275
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-5123765/v1 for Research Square preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG_Cas9-NLS-NES-PP7 was a gift from Wolfgang Wurst (Addgene plasmid # 261212 ; http://n2t.net/addgene:261212 ; RRID:Addgene_261212)

  • For your References section:

    Creating bottom-up RNA transfer vehicles from synthetic protein assemblies. Schuhmacher MK, Gruber C, Lang CMR, Ruijpers RMW, Mazneykova L, Beinsteiner B, Krus A, Tremmel B, Reinhardt F, Kadletz K, Ma Z, Casalta L, Cambra Bort JM, Otify DY, Balken I, Hetzel L, Merl-Pham J, Dorn T, Luchner M, Bauersachs L, Ganea K, Wieser N, Emrich A, Yagmur E, Rager K, Sagindykova G, Armbrust N, Geilenkeuser J, Westmeyer GG, Becirovic E, Biel M, Istvanffy R, Vogt Weisenhorn DM, Truong DJ, Theis FJ, Ebert G, Moretti A, Erturk A, Bahr A, Kupatt C, Jasnin M, Klymiuk N, Giesert F, Wurst W. Nature. 2026 Sep 2. doi: 10.1038/s41586-026-10952-3. 10.1038/s41586-026-10952-3 PubMed 42686908
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