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pET30a(+)-RF1-C-mNeptune
(Plasmid #261406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261406 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET30a(+)
  • Backbone manufacturer
    Alexandra Waldherr (Max Planck), BioCat (Heidelberg, Germany)
  • Backbone size w/o insert (bp) 5225
  • Total vector size (bp) 7088
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RF1
  • Alt name
    Release factor 1
  • Alt name
    prfA
  • Insert Size (bp)
    1863
  • Entrez Gene
    prfA (a.k.a. b1211, ECK1199, sueB, uar, ups?)
  • Promoter T7
  • Tag / Fusion Protein
    • Fluorescent protein (FP) and HIS-tag: C-terminal ...TEV-FP-His construct (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid construct cannot be used for commercial purposes. Academic use has to please credit Alexandra Waldherr :)

Benchling link to our map: https://benchling.com/s/seq-tMkVHUT4SEAh2lG09eNo?item_id=seq_kYR2yBU5hQ&m=slm-EWgTIQ1VrwmntYCFulIK

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a(+)-RF1-C-mNeptune was a gift from Andrei Lupas (Addgene plasmid # 261406 ; http://n2t.net/addgene:261406 ; RRID:Addgene_261406)
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