Andrei Lupas Lab: FluoroPURE Plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
261406	pET30a(+)-RF1-C-mNeptune	Expresses RF1 with C-terminal mNeptune (+ HisTag in E. coli), kanamycin 50 µg/ml
261407	pET30a(+)-RF2-C-mCarmine	Expresses RF2 with C-terminal mCarmine (+ HisTag in E. coli), kanamycin 50 µg/ml
261408	pET30a(+)-RF3-C-mKate2	Expresses RF3 with C-terminal mKate2 (+ HisTag in E. coli), kanamycin 50 µg/ml
261409	pET30a(+)-RRF-C-mRaspberry	Expresses RRF with C-terminal mRaspberry (+ HisTag in E. coli), kanamycin 50 µg/ml
261410	pET30a(+)-EFG-C-mTangerine	Expresses EF-G with C-terminal mTangerine (+ HisTag in E. coli), kanamycin 50 µg/ml
261411	pET30a(+)-EFTu-C-mBanana	Expresses EF-Tu with C-terminal mBanana (+ HisTag in E. coli), kanamycin 50 µg/ml
261412	pET30a(+)-EFTs-C-mPapaya	Expresses EF-Ts with C-terminal mPapaya (+ HisTag in E. coli), kanamycin 50 µg/ml
261415	pET30a(+)-IF1-C-mNeonGreen	Expresses IF1 with C-terminal mNeonGreen (+ HisTag in E. coli), kanamycin 50 µg/ml
261416	pET30a(+)-IF2-C-SuperfolderGFP	Expresses IF2 with C-terminal SuperfolderGFP (+ HisTag in E. coli), kanamycin 50 µg/ml
261417	pET30a(+)-IF3-C-miniGFP	Expresses IF3 with C-terminal miniGFP (+ HisTag in E. coli), kanamycin 50 µg/ml
261418	pET30a(+)-MTF-C-mGold2s	Expresses MTF with C-terminal mGold2s (+ HisTag in E. coli), kanamycin 50 µg/ml, MTF = methionyl-tRNA formyltransferase (required for start codon)
261419	pET30a(+)-AK-C-mCerulean	Expresses AK with C-terminal mCerulean2.D3 (+ HisTag in E. coli), kanamycin 50 µg/ml, AK = adenylate kinase (required for ATP regeneration 2 ADP ⇌ ATP + AMP)
261420	pET30a(+)-CK-C-SuperfolderBFP	Expresses CK with C-terminal SuperfolderBFP (+ HisTag in E. coli), kanamycin 50 µg/ml, CK = creatine kinase (required for ATP and GTP regeneration, removes -P from creatine phosphate)
261421	pET30a(+)-NDK-C-Sirius	Expresses CK with C-terminal Sirius (+ HisTag in E. coli), kanamycin 50 µg/ml, NDK = nucleotide kinase (transfers -P groups ADP + GTP ⇌ ATP + GDP)
261422	pET30a(+)-PPiase-C-Surmine	Expresses PPiase with C-terminal Surmine (+ HisTag in E. coli), kanamycin 50 µg/ml, PPiase = inorganic pyrophosphatase (increases RNA yield and helps transcription by removing PP → 2 Pi)
261423	pET30a(+)-T7-C-NanoLuc	Expresses T7 with C-terminal NanoLuc (+ HisTag in E. coli), kanamycin 50 µg/ml, T7 = RNA polymerase (required for RNA transcription)