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pCMV-Flag-SNAP-CaSR-L723R
(Plasmid #261430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261430 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV-Twist
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaSR-L723R
  • Species
    H. sapiens (human)
  • Mutation
    L723R (Leu723Arg)
  • Entrez Gene
    CASR (a.k.a. CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1, hCasR)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag-SNAP (N terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Flag-SNAP-CaSR-L723R was a gift from Caroline Gorvin (Addgene plasmid # 261430 ; http://n2t.net/addgene:261430 ; RRID:Addgene_261430)

  • For your References section:

    An activating calcium-sensing receptor variant with biased signaling reveals a critical residue for Galpha11 coupling. Benson MR, Wyatt RA, Levine MA, Gorvin CM. J Bone Miner Res. 2025 Feb 2;40(2):270-282. doi: 10.1093/jbmr/zjae199. 10.1093/jbmr/zjae199 PubMed 39658204
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