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PB-CT3G-ERP2-SRSF1
(Plasmid #261722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261722 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PB-CT3G-ERP2-MG
  • Backbone size w/o insert (bp) 5966
  • Total vector size (bp) 6713
  • Modifications to backbone
    Barcoded with the following 20-mer: GCTGCGTTTGATGAGTTTGT
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRSF1
  • Alt name
    SF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    747
  • GenBank ID
    NM_006924.5
  • Entrez Gene
    SRSF1 (a.k.a. ASF, NEDFBA, SF2, SF2p33, SFRS1, SRp30a)
  • Promoter TetOn

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.13.566787 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-CT3G-ERP2-SRSF1 was a gift from George Church (Addgene plasmid # 261722 ; http://n2t.net/addgene:261722 ; RRID:Addgene_261722)

  • For your References section:

    Transcriptomic reprogramming screen identifies SRSF1 as a regulator of aging. Plesa AM, Jung S, Wang HH, Omar F, Shadpour M, Buentello DC, Perez-Matos MC, Horwitz N, Cai G, Ngian ZK, Magalhaes C, Wagers AJ, Mair WB, del Sol A, Church GM. Nat Aging 6, 2057–2073 (2026) 10.1038/s43587-026-01234-8
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