pAAV-CT3G-ER-MG
(Plasmid
#261724)
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Purpose(Empty Backbone) All-in-one AAV destination vector for dox-inducible expression of MegaGate-cloned elements (EF-1α-driven Tet-On 3G, AAV2 ITR-flanked)
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Depositing Lab
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Depositing OrganizationHarvard Medical School
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV-CAG-EGFP barcode-3-SV40 polyA
- Backbone size (bp) 5368
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Modifications to backboneReplaced insert with CT3G-ER-MG cassette to create Gateway-compatible all-in-one TetOn vector.
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Vector typeMammalian Expression, AAV, Gateway Cloning
- Promoter TetOn
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.13.566787 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CT3G-ER-MG was a gift from George Church (Addgene plasmid # 261724 ; http://n2t.net/addgene:261724 ; RRID:Addgene_261724) -
For your References section:
Transcriptomic reprogramming screen identifies SRSF1 as a regulator of aging. Plesa AM, Jung S, Wang HH, Omar F, Shadpour M, Buentello DC, Perez-Matos MC, Horwitz N, Cai G, Ngian ZK, Magalhaes C, Wagers AJ, Mair WB, del Sol A, Church GM. Nat Aging 6, 2057–2073 (2026) 10.1038/s43587-026-01234-8