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pAAV-CT3G-ER-MG
(Plasmid #261724)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261724 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pAAV-CAG-EGFP barcode-3-SV40 polyA
  • Backbone size (bp) 5368
  • Modifications to backbone
    Replaced insert with CT3G-ER-MG cassette to create Gateway-compatible all-in-one TetOn vector.
  • Vector type
    Mammalian Expression, AAV, Gateway Cloning
  • Promoter TetOn

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.13.566787 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CT3G-ER-MG was a gift from George Church (Addgene plasmid # 261724 ; http://n2t.net/addgene:261724 ; RRID:Addgene_261724)
  • For your References section:

    Transcriptomic reprogramming screen identifies SRSF1 as a regulator of aging. Plesa AM, Jung S, Wang HH, Omar F, Shadpour M, Buentello DC, Perez-Matos MC, Horwitz N, Cai G, Ngian ZK, Magalhaes C, Wagers AJ, Mair WB, del Sol A, Church GM. Nat Aging 6, 2057–2073 (2026) 10.1038/s43587-026-01234-8