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pS60-ALFA-cpHalo179-ALFA-7xHis
(Plasmid #261833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261833 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Expression vector pTEV17
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cpHalo179
  • Species
    Synthetic
  • Promoter T7
  • Tags / Fusion Proteins
    • ALFA-tag (N terminal on insert)
    • ALFA-tag (C terminal on insert)
    • 7xHis (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.08.18.745627 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS60-ALFA-cpHalo179-ALFA-7xHis was a gift from Shaohe Wang (Addgene plasmid # 261833 ; http://n2t.net/addgene:261833 ; RRID:Addgene_261833)

  • For your References section:

    WHaloForce enables chemigenetic imaging of molecular tension in living cells and animals. Wu D, Morales EA, Lee J, Pangeni S, Farrants H, Hutchings KA, Barndt RJ, Yu Q, Li X, Shroff H, Wu H, Tebo AG, Lavis LD, Schreiter, Ha ERT, Wang S. bioRxiv 2026.08.18.745627 10.64898/2026.08.18.745627
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