WHaloForce enables chemigenetic imaging of molecular tension in living cells and animals
Wu D, Morales EA, Lee J, Pangeni S, Farrants H, Hutchings KA, Barndt RJ, Yu Q, Li X, Shroff H, Wu H, Tebo AG, Lavis LD, Schreiter, Ha ERT, Wang S
bioRxiv 2026.08.18.745627
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 261833 | pS60-ALFA-cpHalo179-ALFA-7xHis | Expression of cpHalo179 protein with ALFA tag on both termini and 7xHis tag on the C-terminus |
| 261834 | pS61-ALFA-cpWHalo179-ALFA-7xHis | Expression of cpWHalo179 protein with ALFA tag on both termini and 7xHis tag on the C-terminus |
| 261835 | pD185-lenti-pEF1s-Vin-WF | Lentiviral packaging and expression of Vin-WF in mammalian cells |
| 261836 | pD186-lenti-pEF1s-Vin-cpH | Lentiviral packaging and expression of Vin-cpH in mammalian cells |
| 261837 | pD188-lenti-pEF1s-Vin-N-WF | Lentiviral packaging and expression of Vin-N-WF in mammalian cells |
| 261838 | pD190-lenti-pEF1s-Ecad-WF | Lentiviral packaging and expression of Ecad-WF in mammalian cells |
| 261839 | pD191-lenti-pEF1s-Ecad-cpH | Lentiviral packaging and expression of Ecad-cpH in mammalian cells |
| 261840 | pD193-lenti-pEF1s-Ecad-C-WF | Lentiviral packaging and expression of Ecad-C-WF in mammalian cells |
| 261841 | pD161-lenti-pEF1s-aCat-WF-IRES-NLS-mTq2 | Lentiviral packaging and expression of aCat-WF with a nuclear mTurquoise2 reporter in mammalian cells |
| 261842 | pD162-lenti-pEF1s-aCat-cpH-IRES-NLS-mTq2 | Lentiviral packaging and expression of aCat-cpH with a nuclear mTurquoise2 reporter in mammalian cells |
| 261843 | pD163-lenti-pEF1s-aCat-C-WF-IRES-NLS-mTq2 | Lentiviral packaging and expression of aCat-C-WF with a nuclear mTurquoise2 reporter in mammalian cells |
| 261844 | pD178-lenti-pEF1s-cpHalo179-P2A-mTq2 | Lentiviral packaging and expression of cpHalo179 with an mTurquoise2 reporter in mammalian cells |
| 261845 | pD179-lenti-pEF1s-WHalo179-P2A-mTq2 | Lentiviral packaging and expression of cpWHalo179 with an mTurquoise2 reporter in mammalian cells |
| 261846 | pD124-lenti-pEF1s-HaloTag-P2A-mTq2 | Lentiviral packaging and expression of HaloTag with an mTurquoise2 reporter in mammalian cells |