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pCMV-rox-STOP-rox-LifeAct-mCherry
(Plasmid #261896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261896 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech/Takara
  • Backbone size w/o insert (bp) 4898
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct
  • Alt name
    LifeAct-mCherry
  • Species
    Synthetic
  • Mutation
    None
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

rox-flanked STOP cassette (RSR) upstream of the F-actin-binding peptide Lifeact fused to mCherry, so expression occurs only after Dre recombination.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-rox-STOP-rox-LifeAct-mCherry was a gift from Frank Bradke (Addgene plasmid # 261896 ; http://n2t.net/addgene:261896 ; RRID:Addgene_261896)

  • For your References section:

    An intrinsic cytoskeletal oscillator establishes neuronal polarity. Lin TC, Coles CH, Alfadil E, Fassler F, Husch A, Dupraz S, Pietralla T, Narita A, Schelski M, Flynn KC, Stern S, Mohl C, Hilton BJ, Vauti F, Arnold HH, Schur FKM, Bradke F. Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6. 10.1038/s41586-026-10755-6 PubMed 42420447
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