An intrinsic cytoskeletal oscillator establishes neuronal polarity.

Lin TC, Coles CH, Alfadil E, Fassler F, Husch A, Dupraz S, Pietralla T, Narita A, Schelski M, Flynn KC, Stern S, Mohl C, Hilton BJ, Vauti F, Arnold HH, Schur FKM, Bradke F
Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
261896	pCMV-rox-STOP-rox-LifeAct-mCherry	Dre-dependent F-actin reporter.Used for in utero electroporation to label F-actin in cortical neurons.
261899	pTalpha1-Vika	Neuron-specific expression of Vika recombinase under the tubulin alpha-1 promoter. It is used for in utero electroporation in the developing cortex.
261903	pCMV-Myl12a-SNAP	SNAP-tagged myosin regulatory light chain for live-cell labelling with SiR-benzylguanine. Used to follow actomyosin condensation and dispersion during neurite retraction and extension.
261906	pCMV-KIF5C(1-560)-SNAP	Truncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused to SNAP, used as a marker of nascent axons in cultured neurons.
261907	pCMV-KIF5C(1-560)-mNeonGreen-Zdk1	Truncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused tomNeonGreen, used as a marker of nascent axons in cultured neurons.
261908	pCMV-Myl12a-mNeonGreen-Zdk1	mNeonGreen-tagged myosin regulatory light chain for live-cell labelling. Used to follow actomyosin condensation and dispersion during neurite retraction and extension.