An intrinsic cytoskeletal oscillator establishes neuronal polarity.
Lin TC, Coles CH, Alfadil E, Fassler F, Husch A, Dupraz S, Pietralla T, Narita A, Schelski M, Flynn KC, Stern S, Mohl C, Hilton BJ, Vauti F, Arnold HH, Schur FKM, Bradke F
Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 261894 | pCAG-vox-STOP-vox-ZsGreen | Vika-dependent cytoplasmic reporter. vox-flanked STOP cassette (VSV) upstream of ZsGreen, so expression occurs only after Vika recombination. Used for in utero electroporation. |
| 261895 | pCAG-rox-STOP-rox-LifeAct-mNeonGreen | Dre-dependent F-actin reporter. Used for in utero electroporation to label F-actin in cortical neurons. |
| 261896 | pCMV-rox-STOP-rox-LifeAct-mCherry | Dre-dependent F-actin reporter.Used for in utero electroporation to label F-actin in cortical neurons. |
| 261897 | pCAG-rox-STOP-rox-LynTM-mNeonGreen | Dre-dependent membrane reporter. Used to outline neuronal morphology in acute cortical slices. |
| 261898 | pCAG-lox-STOP-lox-LynTM-mNeonGreen | Cre-dependent membrane reporter. lox-flanked STOP cassette (LSL) upstream of the LYN membrane-targeting motif fused to mNeonGreen, so expression occurs only after Cre recombination. |
| 261899 | pTalpha1-Vika | Neuron-specific expression of Vika recombinase under the tubulin alpha-1 promoter. It is used for in utero electroporation in the developing cortex. |
| 261900 | pBetaActin-Arp3-SNAP | SNAP-tagged ARP2/3 complex subunit ARP3 for live-cell labelling with SiR-benzylguanine. Used to follow ARP2/3 distribution and intensity fluctuations. |
| 261901 | pBetaActin-Arp3-EGFP | Functional rescue construct: pCAG-driven ARP3-EGFP. Restores neurite length and axon formation when transfected into Actr3 knockout neurons. |
| 261902 | pBetaActin-Arp3-mEos3.2 | Photoconvertible ARP3 fusion. Used to measure the turnover of somatic ARP3 by photoconversion. |
| 261903 | pCMV-Myl12a-SNAP | SNAP-tagged myosin regulatory light chain for live-cell labelling with SiR-benzylguanine. Used to follow actomyosin condensation and dispersion during neurite retraction and extension. |
| 261904 | pBetaActin-Arp3-mNeonGreen | mNeonGreen-tagged ARP3, used for the reciprocal-label triple imaging of F-actin, myosin II and ARP2/3 (with Lifeact-mScarlet and MRLC-SNAP-SiR). |
| 261905 | pBetaActin-EB3-SNAP | SNAP-tagged EB3 microtubule plus-end marker for live-cell labelling with SiR-benzylguanine. |
| 261906 | pCMV-KIF5C(1-560)-SNAP | Truncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused to SNAP, used as a marker of nascent axons in cultured neurons. |
| 261907 | pCMV-KIF5C(1-560)-mNeonGreen-Zdk1 | Truncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused tomNeonGreen, used as a marker of nascent axons in cultured neurons. |
| 261908 | pCMV-Myl12a-mNeonGreen-Zdk1 | mNeonGreen-tagged myosin regulatory light chain for live-cell labelling. Used to follow actomyosin condensation and dispersion during neurite retraction and extension. |