pCMV-KIF5C(1-560)-mNeonGreen-Zdk1
(Plasmid
#261907)
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PurposeTruncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused tomNeonGreen, used as a marker of nascent axons in cultured neurons.
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Depositing Lab
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Depositing OrganizationDeutsches Zentrum fuer Neurodegenerative Erkrankungen (DZNE)
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 4046
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKif5c
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Alt nameKIF5C(1-560)
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Alt namekinesin family member 5C
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SpeciesR. norvegicus (rat)
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MutationNone
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Entrez GeneKif5c
- Promoter CMV
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Tag
/ Fusion Protein
- mNeonGreen; Zdk1 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byKristen Verhey (KIF5C(1-560) insert sequence sourced from Addgene Plasmid #61676)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-KIF5C(1-560)-mNeonGreen-Zdk1 was a gift from Frank Bradke (Addgene plasmid # 261907 ; http://n2t.net/addgene:261907 ; RRID:Addgene_261907) -
For your References section:
An intrinsic cytoskeletal oscillator establishes neuronal polarity. Lin TC, Coles CH, Alfadil E, Fassler F, Husch A, Dupraz S, Pietralla T, Narita A, Schelski M, Flynn KC, Stern S, Mohl C, Hilton BJ, Vauti F, Arnold HH, Schur FKM, Bradke F. Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6. 10.1038/s41586-026-10755-6 PubMed 42420447