pCMV-KIF5C(1-560)-SNAP
(Plasmid #261906)

• Purpose: Truncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused to SNAP, used as a marker of nascent axons in cultured neurons.
• Depositing Lab: Frank Bradke
• Depositing Organization: Deutsches Zentrum fuer Neurodegenerative Erkrankungen (DZNE)
  Located in Germany
• Publication: Lin et al Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech/Takara
• Backbone size w/o insert (bp): 4095
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif5c
• Alt name: KIF5C(1-560)
• Alt name: kinesin family member 5C
• Species: R. norvegicus (rat)
• Insert Size (bp): 1677
• Mutation: None
• Entrez Gene: Kif5c
• Promoter: CMV
• Tag / Fusion Protein:
  • SNAP-tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kristen Verhey (KIF5C(1-560) insert sequence sourced from Addgene Plasmid #61676)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry