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pBetaActin-EB3-SNAP
(Plasmid #261905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261905 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCAG (pBeta-actin)
  • Backbone size w/o insert (bp) 6119
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPRE3
  • Alt name
    EB3
  • Alt name
    microtubule end binding protein 3
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Promoter Chicken beta-actin
  • Tag / Fusion Protein
    • SNAP-tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Frank Bradke (EB3 insert sequence sourced from Addgene Plasmid #196848)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to image microtubule compression and the appearance of microtubule kinks on ARP2/3 inhibition with CK-666, and their release on washout.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBetaActin-EB3-SNAP was a gift from Frank Bradke (Addgene plasmid # 261905 ; http://n2t.net/addgene:261905 ; RRID:Addgene_261905)

  • For your References section:

    An intrinsic cytoskeletal oscillator establishes neuronal polarity. Lin TC, Coles CH, Alfadil E, Fassler F, Husch A, Dupraz S, Pietralla T, Narita A, Schelski M, Flynn KC, Stern S, Mohl C, Hilton BJ, Vauti F, Arnold HH, Schur FKM, Bradke F. Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6. 10.1038/s41586-026-10755-6 PubMed 42420447
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