pCMV-Myl12a-SNAP
(Plasmid
#261903)
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PurposeSNAP-tagged myosin regulatory light chain for live-cell labelling with SiR-benzylguanine. Used to follow actomyosin condensation and dispersion during neurite retraction and extension.
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Depositing Lab
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Depositing OrganizationDeutsches Zentrum fuer Neurodegenerative Erkrankungen (DZNE)
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepSNAPf-N1 (pEGFP-N1 derived)
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyl12a
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Alt nameMRLC
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Alt namemyosin regulatory light chain 12A
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SpeciesM. musculus (mouse)
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MutationNone
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Entrez GeneMyl12a (a.k.a. 2900073G15Rik)
- Promoter CMV
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Tag
/ Fusion Protein
- SNAP-tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Myl12a-SNAP was a gift from Frank Bradke (Addgene plasmid # 261903 ; http://n2t.net/addgene:261903 ; RRID:Addgene_261903) -
For your References section:
An intrinsic cytoskeletal oscillator establishes neuronal polarity. Lin TC, Coles CH, Alfadil E, Fassler F, Husch A, Dupraz S, Pietralla T, Narita A, Schelski M, Flynn KC, Stern S, Mohl C, Hilton BJ, Vauti F, Arnold HH, Schur FKM, Bradke F. Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6. 10.1038/s41586-026-10755-6 PubMed 42420447