pBetaActin-Arp3-mNeonGreen
(Plasmid #261904)

• Purpose: mNeonGreen-tagged ARP3, used for the reciprocal-label triple imaging of F-actin, myosin II and ARP2/3 (with Lifeact-mScarlet and MRLC-SNAP-SiR).
• Depositing Lab: Frank Bradke
• Depositing Organization: Deutsches Zentrum fuer Neurodegenerative Erkrankungen (DZNE)
  Located in Germany
• Publication: Lin et al Nature. 2026 Jul 8. doi: 10.1038/s41586-026-10755-6.

Backbone

• Vector backbone: pCAG (pBeta-actin)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACTR3
• Alt name: Arp3
• Alt name: actin related protein 3
• Species: H. sapiens (human)
• Mutation: None
• Entrez Gene: ACTR3 (a.k.a. ARP3)
• Promoter: Chicken beta-actin
• Tag / Fusion Protein:
  • mNeonGreen (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christien Merrifield (ACTR3 insert sequence sourced from Addgene Plasmid #27682)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry