pLentiCRISPRV2-murineSlc19a1
(Plasmid
#261918)
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PurposeUse to generate Slc19a1 knockout
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Depositing Lab
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Depositing OrganizationBoston Children's Hospital
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLentiCRISPRV2
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Backbone manufacturerFeng Zhang (Addgene #52961)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSlc19a1
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gRNA/shRNA sequenceTCTTTCTAAAGCGCCCTAAG
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SpeciesM. musculus (mouse)
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Entrez GeneSlc19a1 (a.k.a. RFC, RFC-1, RFC1)
- Promoter EFS-NS
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiCRISPRV2-murineSlc19a1 was a gift from Jonathan Kagan (Addgene plasmid # 261918 ; http://n2t.net/addgene:261918 ; RRID:Addgene_261918) -
For your References section:
Purine and pyrimidine-based bacterial cyclic dinucleotides egress the phagosome and activate the innate immune sensor STING. Ragland SA, Lindblad KE, Li Y, Choi JH, Okin D, Mao JQ, Richmond-Buccola D, Coronado DR, Brown MR, Snapper SB, Kranzusch PJ, Kagan JC. Immunity. 2026 Aug 11;59(8):2086-2105.e17. doi: 10.1016/j.immuni.2026.06.023. Epub 2026 Jul 17. 10.1016/j.immuni.2026.06.023 PubMed 42468529