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pcDNA3.1-DEST-hCIC-L-W946A-V5
(Plasmid #262257)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262257 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 262257-D50 50 μg of DNA in Tris buffer $495
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DNA 262257-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1-DEST
  • Total vector size (bp) 13099
  • Modifications to backbone
    Derived from pcDNA3.1-3xFLAG-DEST, with stop codons upstream of the 3xFLAG to prevent FLAG expression.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Capicua-Long isoform
  • Alt name
    CIC-L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7596
  • Mutation
    W946A (ATXN1/1L binding domain mutant)
  • GenBank ID
    NM_001304815.2
  • Entrez Gene
    CIC (a.k.a. MRD45)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-DEST-hCIC-L-W946A-V5 was a gift from Huda Zoghbi (Addgene plasmid # 262257 ; http://n2t.net/addgene:262257 ; RRID:Addgene_262257)

  • For your References section:

    Functional divergence of Capicua isoforms explains differential tissue vulnerability in neurological disease. Lee H, Gonzalez EV, Rivera EM, Durham MA, Richman R, Chu EH, Xia K, Chen H, Liu Z, Veeraragavan S, Shivanna B, Zoghbi HY. Genes Dev. 2026 Aug 3;40(15-16):1267-1282. doi: 10.1101/gad.353596.125. 10.1101/gad.353596.125 PubMed 42476817
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