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pcDNA3.1-DEST-hCIC-S-W37A-V5
(Plasmid #262258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262258 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1-DEST
  • Total vector size (bp) 10363
  • Modifications to backbone
    Derived from pcDNA3.1-3xFLAG-DEST, with stop codons upstream of the 3xFLAG to prevent FLAG expression.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Capicua-Short isoform
  • Alt name
    CIC-S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4869
  • Mutation
    W37A (ATXN1/1L binding domain mutant)
  • GenBank ID
    NM_015125.5
  • Entrez Gene
    CIC (a.k.a. MRD45)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-DEST-hCIC-S-W37A-V5 was a gift from Huda Zoghbi (Addgene plasmid # 262258 ; http://n2t.net/addgene:262258 ; RRID:Addgene_262258)

  • For your References section:

    Functional divergence of Capicua isoforms explains differential tissue vulnerability in neurological disease. Lee H, Gonzalez EV, Rivera EM, Durham MA, Richman R, Chu EH, Xia K, Chen H, Liu Z, Veeraragavan S, Shivanna B, Zoghbi HY. Genes Dev. 2026 Aug 3;40(15-16):1267-1282. doi: 10.1101/gad.353596.125. 10.1101/gad.353596.125 PubMed 42476817
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