pcDNA3.1-Flag-hATXN1
(Plasmid
#262259)
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PurposeExpresses ATXN1 in mammalian cells
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Depositing Lab
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Depositing OrganizationBaylor College of Medicine
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 7859
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtaxin-1
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Alt nameATXN1
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SpeciesH. sapiens (human)
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Insert Size (bp)2472
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GenBank IDNM_000332.4
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Entrez GeneATXN1 (a.k.a. ATX1, D6S504E, SCA1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Flag-hATXN1 was a gift from Huda Zoghbi (Addgene plasmid # 262259 ; http://n2t.net/addgene:262259 ; RRID:Addgene_262259) -
For your References section:
Functional divergence of Capicua isoforms explains differential tissue vulnerability in neurological disease. Lee H, Gonzalez EV, Rivera EM, Durham MA, Richman R, Chu EH, Xia K, Chen H, Liu Z, Veeraragavan S, Shivanna B, Zoghbi HY. Genes Dev. 2026 Aug 3;40(15-16):1267-1282. doi: 10.1101/gad.353596.125. 10.1101/gad.353596.125 PubMed 42476817