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V5-RavZ C268A
(Plasmid #262586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262586 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCDH
  • Backbone size w/o insert (bp) 7403
  • Total vector size (bp) 8954
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RavZ
  • Insert Size (bp)
    1545
  • Mutation
    changed cysteine 268 to alanine
  • Entrez Gene
    ravZ (a.k.a. AVR58_RS08600, AVR58_08395)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thomas Melia, Addgene plasmid 250878

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V5-RavZ C268A was a gift from Xiaojun Tan (Addgene plasmid # 262586 ; http://n2t.net/addgene:262586 ; RRID:Addgene_262586)

  • For your References section:

    A TBK1-independent primordial function of STING in lysosomal biogenesis. Lv B, Dion WA, Yang H, Xun J, Kim DH, Zhu B, Tan JX. Mol Cell. 2024 Oct 17;84(20):3979-3996.e9. doi: 10.1016/j.molcel.2024.08.026. Epub 2024 Sep 19. 10.1016/j.molcel.2024.08.026 PubMed 39423796
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