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(Plasmid #26305)


Item Catalog # Description Quantity Price (USD)
Plasmid 26305 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 5549
  • Vector type
    Worm Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use DB3.1 E. coli (must be ccdB resistant). Note that ccdB Survival bacteria (Invitrogen) do not work for this plasmid.
  • Copy number
    Low Copy


  • Gene/Insert name

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AttR4 (destroyed during cloning)
  • 3′ cloning site AttR3 (destroyed during cloning)
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 (-21) Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene). Zeocin resistance gene from pcDNA4(Invitrogen).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use for phleomycin selection of transgenes in C. elegans.
The PuroR gene in pBCN21-R4R3 was replaced by the ZeocinR gene for phleomycin resistance.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBCN25-R4R3 was a gift from Ben Lehner (Addgene plasmid # 26305 ; ; RRID:Addgene_26305)
  • For your References section:

    Rapid selection of transgenic C. elegans using antibiotic resistance. Semple JI, Garcia-Verdugo R, Lehner B. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1495 PubMed 20729840