|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26301||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5879
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsUse DB3.1 E. coli (must be ccdB resistant). Note that ccdB Survival bacteria (Invitrogen) do not work for this plasmid.
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ cloning site AttR4 (destroyed during cloning)
- 3′ cloning site AttR3 (destroyed during cloning)
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 (-21) Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPuromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).
Terms and Licenses
Use for puromycin selection of transgenes in C. elegans and related species if transgene has a visual phenotype and Pmyo-2::mCherry expression is undesirable.
The unc-119 gene in pCG150 was replaced by the Prpl-28::PuroR::let-858_3'UTR cassette for puromycin selection in worms.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBCN21-R4R3 was a gift from Ben Lehner (Addgene plasmid # 26301 ; http://n2t.net/addgene:26301 ; RRID:Addgene_26301)
For your References section:Rapid selection of transgenic C. elegans using antibiotic resistance. Semple JI, Garcia-Verdugo R, Lehner B. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1495 PubMed 20729840