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(Plasmid #26348)


Item Catalog # Description Quantity Price (USD)
Plasmid 26348 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Bacterial promoter::PuroR::bacterial 3p flank
  • Alt name
    Puromycin N-acetyl-tranferase (PAC)
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Based on pPD49.78 vector (Addgene). Puromycin resistance gene from pBabePuro (Addgene). 5' and 3' sequences flanking the AmpR gene were placed upstream and downstream of PuroR to confer bacterial expression

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transform this plasmid into E. coli and use as a food source for worms grown on puromycin selective plates.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pbacPuroR was a gift from Ben Lehner (Addgene plasmid # 26348 ; ; RRID:Addgene_26348)
  • For your References section:

    Generating transgenic nematodes by bombardment and antibiotic selection. Semple JI, Biondini L, Lehner B. Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864. 10.1038/nmeth.1864 PubMed 22290182