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pBacPuroR-NeoR
(Plasmid #34921)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34921 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD49.78
  • Backbone size w/o insert (bp) 3300
  • Modifications to backbone
    PuroR and NeoR under the control of 5' and 3' bacterial elements taken from up and downstream of the Ampicillin resistance gene.
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418), Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Bacterial promoter::PuroR::bacterial 3p flank
  • Alt name
    Puromycin N-acetyl-tranferase (PAC)
  • Species
    Streptomyces
  • Insert Size (bp)
    979

Gene/Insert 2

  • Gene/Insert name
    Bacterial promoter::NeoR::bacterial 3p flank
  • Alt name
    neo
  • Species
    Tn5 transposon
  • Insert Size (bp)
    1170

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Based on pPD49.78 vector (Addgene). Puromycin resistance gene from pBabePuro (Addgene). 5' and 3' sequences flanking the AmpR gene were placed upstream and downstream of PuroR and NeoR to confer bacterial expression
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

Transform this plasmid into E. coli and use as a food source for worms grown on puromycin & G418 selective plates.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBacPuroR-NeoR was a gift from Ben Lehner (Addgene plasmid # 34921 ; http://n2t.net/addgene:34921 ; RRID:Addgene_34921)
  • For your References section:

    Generating transgenic nematodes by bombardment and antibiotic selection. Semple JI, Biondini L, Lehner B. Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864. 10.1038/nmeth.1864 PubMed 22290182