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pbacNeoR
(Plasmid #26349)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26349 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD49.78
  • Backbone size w/o insert (bp) 3306
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bacterial promoter::NeoR::bacterial 3p flank
  • Alt name
    neo
  • Species
    Tn5 transposon
  • Insert Size (bp)
    1170

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from pPD49.78 vector (Addgene). Neomycin resistance ORF from pEGFP-C1 (Addgene). 5' and 3' sequences flanking the AmpR gene were placed upstream and downstream of NeoR to confer bacterial expression.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transform this plasmid into E. coli and use as a food source for worms grown on G418 selective plates.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pbacNeoR was a gift from Ben Lehner (Addgene plasmid # 26349 ; http://n2t.net/addgene:26349 ; RRID:Addgene_26349)
  • For your References section:

    Generating transgenic nematodes by bombardment and antibiotic selection. Semple JI, Biondini L, Lehner B. Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864. 10.1038/nmeth.1864 PubMed 22290182