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pRSET B_ALFAblock-4
(Plasmid #263099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263099 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSET B
  • Backbone manufacturer
    ThermoFisher Invitrogen
  • Backbone size w/o insert (bp) 2754
  • Total vector size (bp) 4011
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    SHuffle T7 E. coli cells (New England Biolabs) recommended for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALFAblock-4
  • Insert Size (bp)
    1257
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • T7 tag (N terminal on insert)
    • Xpress tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.28.685015 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET B_ALFAblock-4 was a gift from Peter Dedecker (Addgene plasmid # 263099 ; http://n2t.net/addgene:263099 ; RRID:Addgene_263099)

  • For your References section:

    NanoBlocks: creating genetically-encoded protein switches using competitive binding. Denis S, Van Deuren V, Frecot DI, Rothbauer U, Ismail S, Tebo AG, Dedecker P. bioRxiv 2025.10.28.685015 10.1101/2025.10.28.685015
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