NanoBlocks: creating genetically-encoded protein switches using competitive binding
Denis S, Van Deuren V, Frecot DI, Rothbauer U, Ismail S, Tebo AG, Dedecker P
bioRxiv 2025.10.28.685015
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 263099 | pRSET B_ALFAblock-4 | For bacterial expression of a fluorescent biosensor recognizing the ALFA-tag peptide. |
| 263100 | pRSET B_ePDZblock-480-T | For bacterial expression of a fluorescent biosensor recognizing a short peptide (TGWETWV) with high affinity (K'd = 490 nM). |
| 263104 | pRSET B_ALFAblam-4 | For bacterial expression of a luminescent biosensor recognizing the ALFA-tag peptide. |
| 263105 | pcDNA5/FRT/TO_darkmCherry-IRES-HaloTag7 | For mammalian expression of non-fluorescent (dark) mCherry and HaloTag7. |
| 263106 | pcDNA5/FRT/TO_darkmCherry-TGWETWV-IRES-HaloTag7 | For mammalian expression of non-fluorescent (dark) mCherry C-terminally tagged with the TGWETWV peptide, and HaloTag7. |