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pcDNA5/FRT/TO_darkmCherry-IRES-HaloTag7
(Plasmid #263105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263105 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    ThermoFisher Invitrogen
  • Backbone size w/o insert (bp) 5140
  • Total vector size (bp) 7308
  • Modifications to backbone
    IRES placed in between dark mCherry and HaloTag7
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dark mCherry
  • Alt name
    non-fluorescent mCherry
  • Alt name
    mCherry Y72N
  • Insert Size (bp)
    708
  • Mutation
    mCherry with mutation Y72N, which inactivates the chromophore and renders it non-fluorescent
  • Promoter CMV/TO

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    HaloTag7
  • Insert Size (bp)
    891
  • Promoter CMV/TO

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pcDNA5/FRT/TO backbone is designed for use with the Flp-In T-REx system (Invitrogen). However, it acts as a regular pcDNA3.1 backbone when transfected into unmodified mammalian lab cell lines.

Please visit https://doi.org/10.1101/2025.10.28.685015 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO_darkmCherry-IRES-HaloTag7 was a gift from Peter Dedecker (Addgene plasmid # 263105 ; http://n2t.net/addgene:263105 ; RRID:Addgene_263105)

  • For your References section:

    NanoBlocks: creating genetically-encoded protein switches using competitive binding. Denis S, Van Deuren V, Frecot DI, Rothbauer U, Ismail S, Tebo AG, Dedecker P. bioRxiv 2025.10.28.685015 10.1101/2025.10.28.685015
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