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dACE2-GFP
(Plasmid #263185)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263185 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.4
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 6011
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dACE2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2103
  • GenBank ID
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Promoter CMV
  • Tag / Fusion Protein
    • TurboGFP (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dACE2-GFP was a gift from Ludmila Prokunina-Olsson (Addgene plasmid # 263185 ; http://n2t.net/addgene:263185 ; RRID:Addgene_263185)

  • For your References section:

    Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor. Onabajo OO, Banday AR, Stanifer ML, Yan W, Obajemu A, Santer DM, Florez-Vargas O, Piontkivska H, Vargas JM, Ring TJ, Kee C, Doldan P, Tyrrell DL, Mendoza JL, Boulant S, Prokunina-Olsson L. Nat Genet. 2020 Dec;52(12):1283-1293. doi: 10.1038/s41588-020-00731-9. Epub 2020 Oct 19. 10.1038/s41588-020-00731-9 PubMed 33077916
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