A3G5.13P (spCas9)
(Plasmid
#263230)
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PurposeMammalian expression of the A3G5.13P (A3G5.13-PAP) engineered base editor variant using spCas9. CMV promoter. AmpR marker for E. coli.
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Depositing Lab
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Depositing OrganizationUniversity of Pennsylvania
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneAmpR
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA3G5.13P (spCas9)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
A3G5.13P (spCas9) was a gift from Xue Gao (Addgene plasmid # 263230 ; http://n2t.net/addgene:263230 ; RRID:Addgene_263230) -
For your References section:
Precision A3G base editors for disease modeling and correction. Zeng H, Liu A, Daniel TC, Golla DA, Lu Z, Serodio R, Millette BA, Lingineni A, Gilberd P, Chee K, Talloo K, Peddi A, Park SH, Bao G, Gao X. Mol Ther. 2026 Apr 1;34(4):2343-2360. doi: 10.1016/j.ymthe.2026.01.003. Epub 2026 Jan 14. 10.1016/j.ymthe.2026.01.003 PubMed 41536070
