pRecA-YFP-CFP
(Plasmid
#263233)
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PurposerecA promoter driving expression of YFP reporter and constitutive expression of CFP reporter
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepZS4*5noO1+11-CFP
- Backbone size w/o insert (bp) 4443
- Total vector size (bp) 5447
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlso resistant to streptomycin
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameYFP
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SpeciesSynthetic
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Insert Size (bp)717
- Promoter recA
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cttcaccctctccactgacag
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byYFP and backbone was received from Robert Brewster from University of Massachusetts Worcester
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRecA-YFP-CFP was a gift from Amir Mitchell (Addgene plasmid # 263233 ; http://n2t.net/addgene:263233 ; RRID:Addgene_263233) -
For your References section:
Colibactin-induced damage in bacteria is cell contact independent. Lowry E, Mitchell A. mBio. 2025 Jan 8;16(1):e0187524. doi: 10.1128/mbio.01875-24. Epub 2024 Nov 22. 10.1128/mbio.01875-24 PubMed 39576109