pCAG-VPS13A-3xFLAG
(Plasmid
#263522)
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PurposeExpress full-length, codon-optimized, human VPS13A with a C-terminal 3xFLAG tag in mammalian expression systems
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Depositing Lab
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Depositing OrganizationYale University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-VPS13A-3xFLAG was a gift from Karin Reinisch (Addgene plasmid # 263522 ; http://n2t.net/addgene:263522 ; RRID:Addgene_263522) -
For your References section:
Mechanism of lipid transfer by bridge-like protein VPS13A and the scramblase XK. Hu B, Alvarez D, Rocha-Roa C, Guyard V, Li D, Ahmed Y, Wang X, De Camilli P, Vanni S, Reinisch KM. Cell. 2026 Aug 6;189(16):5135-5149.e6. doi: 10.1016/j.cell.2026.05.027. Epub 2026 Jun 12. 10.1016/j.cell.2026.05.027 PubMed 42285089