pET28a-IDP-TAG-CHis6
(Plasmid #264002)

• Purpose: E. coli expression of FUS-derived IDP containing amber codon for site-specific DOPA incorporation and antifouling surface coating.
• Depositing Lab: Han Xiao
• Depositing Organization: Rice University
  Located in the United States of America
• Publication: Zhang et al Small Methods. 2024 Sep 17:e2400230. doi: 10.1002/smtd.202400230.

Backbone

• Vector backbone: pET-28a(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FUS-derived IDP
• Alt name: IDP-FUS
• Species: H. sapiens (human)
• Insert Size (bp): 729
• Mutation: Y33TAG (amber stop codon replacing Tyr33)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Small Methods SI p.11: pET28a(+)-T7-IDP construct using g-block and Gibson (mz88/mz89; mz77/mz78). SI Table S2 confirms Y33TAG and C-terminal ...GHHHHHH*. Confirm precise TAG codon and plasmid map. | MAP QC: IDP Y33TAG verified: map positions 5134-5136 change TAT > TAG. | Complete sequence length: 5935 bp. Sequence from supplied SnapGene map, not confirmed by independent resequencing.