Harnessing Nature-Inspired Catechol Amino Acid to Engineer Sticky Proteins and Bacteria.
Zhang M, Chen Y, Chung A, Yang S, Choi CH, Zhang S, Han Y, Xiao H
Small Methods. 2024 Sep 17:e2400230. doi: 10.1002/smtd.202400230.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 264001 | pET28a-IDP-WT-CHis6 | E. coli expression of wild-type FUS-derived intrinsically disordered protein used as reference for antifouling experiments. |
| 264002 | pET28a-IDP-TAG-CHis6 | E. coli expression of FUS-derived IDP containing amber codon for site-specific DOPA incorporation and antifouling surface coating. |
| 264003 | pEvol-araBAD-OmpA-WT | Arabinose-inducible expression of wild-type E. coli outer-membrane protein A (OmpA) for bacterial adhesion control. |
| 264004 | pEvol-araBAD-OmpA-63TAG64 | Arabinose-inducible expression of OmpA with an amber mutation for site-specific DOPA incorporation and enhanced bacterial surface adhesion. |