pEvol-araBAD-OmpA-WT
(Plasmid #264003)

• Purpose: Arabinose-inducible expression of wild-type E. coli outer-membrane protein A (OmpA) for bacterial adhesion control.
• Depositing Lab: Han Xiao
• Depositing Organization: Rice University
  Located in the United States of America
• Publication: Zhang et al Small Methods. 2024 Sep 17:e2400230. doi: 10.1002/smtd.202400230.

Backbone

• Vector backbone: pEvol
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ompA
• Alt name: outer membrane protein A
• Species: Escherichia coli
• Insert Size (bp): 1059
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Small Methods SI p.11: OmpA g-block amplified mz46/mz47 and pEVOL backbone amplified mz9/mz10, Gibson assembly. SI Table S2 contains OmpA63TAG64 protein and ends QAHHHHHH*. Verify WT variant sequence and 6xHis tag placement in final stock. | MAP QC: OmpA WT with C-terminal His6; OmpA signal peptide and araBAD promoter annotated. | Complete sequence length: 4384 bp. Sequence from supplied SnapGene map, not confirmed by independent resequencing.