pEvol-araBAD-OmpA-63TAG64
(Plasmid #264004)

• Purpose: Arabinose-inducible expression of OmpA with an amber mutation for site-specific DOPA incorporation and enhanced bacterial surface adhesion.
• Depositing Lab: Han Xiao
• Depositing Organization: Rice University
  Located in the United States of America
• Publication: Zhang et al Small Methods. 2024 Sep 17:e2400230. doi: 10.1002/smtd.202400230.

Backbone

• Vector backbone: pEvol
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ompA
• Alt name: outer membrane protein A
• Species: Escherichia coli
• Insert Size (bp): 1062
• Mutation: In-frame amber codon between OmpA residues Tyr63 and Lys64 (OmpA63TAG64)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Small Methods SI Table S2: OmpA63TAG64 protein sequence shows ...AGF GG*YQV...; C-terminal ...QAHHHHHH*. SI construction p.11 PCR/Gibson mz46/mz47 mz9/mz10; verify exact nucleotide, as amino-acid representation is not full plasmid DNA. | MAP QC: Amber TAG inserted between OmpA Y63 and K64, +3 bp relative to #4; C-terminal His6. | Complete sequence length: 4387 bp. Sequence from supplied SnapGene map, not confirmed by independent resequencing.