pHTN-hSOD-A4V-W32TAG
(Plasmid #264007)

• Purpose: Mammalian expression of hSOD1-A4V bearing W32TAG for site-specific AnapTh incorporation to monitor aggregation-associated microenvironment changes.
• Depositing Lab: Han Xiao
• Depositing Organization: Rice University
  Located in the United States of America
• Publication: Yang et al Nat Chem Biol. 2026 Jan;22(1):97-108. doi: 10.1038/s41589-025-02003-1. Epub 2025 Sep 11.

Backbone

• Vector backbone: pHTN
• Vector type: Mammalian Expression
• Selectable markers: Neomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOD1
• Alt name: human superoxide dismutase 1
• Species: H. sapiens (human)
• Insert Size (bp): 498
• Mutation: A4V; W32TAG
• Promoter: CMV

Cloning Information

• Cloning method: Other

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NCB Methods: site-directed W32TAG from pHTN-hSOD1-A4V; SI Table 3 primers mz521/mz522. Confirm if C-terminal His6 is present; NCB Methods describe independent His6 addition with mz773/mz774. | MAP QC: W32 codon annotated TAG; EXACT same full nucleotide sequence as #7. SnapGene topology indicates LINEAR; verify physical plasmid circularity. | Complete sequence length: 5603 bp. Sequence from supplied SnapGene map, not confirmed by independent resequencing.