pdestMB14
(Plasmid #26415)

• Depositing Lab: Marc Vidal
• Publication: Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Backbone

• Vector backbone: pDP#MM016b
• Backbone size w/o insert (bp): 8623
• Vector type: Bacterial Expression, Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB resistant bacteria Ex: DB3.1 E. coli
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: attR4-ccdB-attR2
• Insert Size (bp): 3479

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow.

The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.